Last season's EFL Cup finalists Southampton have been drawn against Wolverhampton Wanderers in the second round of the 2017-18 competition.
The Saints, who were defeated by Manchester United in last term's final, have been handed a home tie against the Championship outfit in the South Section of the draw.
Every Premier League club that is not involved in European competition also entered the competition in round two, with Crystal Palace drawing Ipswich Town away, Watford paying a visit to Bristol City and newly-promoted Brighton & Hove Albion hosting Barnet of League Two.
Other highlights from the South Section include West Ham United's visit to Cheltenham Town, Swansea City away to MK Dons, and Bournemouth away to Birmingham City.
The pick of the ties in the North Section is a Lancashire derby between Blackburn Rovers and Burnley, with the former at home.
Newly-promoted Huddersfield Town will host Rotherham United, Leicester City are at Sheffield United and West Bromwich Albion will visit League Two Accrington Stanley.
The ties will be played during the week commencing August 21.
The draw in full:
South Section
Brentford vs. QPR
Ipswich vs. Crystal Palace
Bristol City vs. Watford
Charlton vs. Norwich
Cheltenham vs. West Ham
Brighton vs. Barnet
Cardiff vs. Burton Albion
Southampton vs. Wolves
Fulham vs. Bristol Rovers
MK Dons vs. Swansea
Birmingham vs. Bournemouth
Reading vs. Millwall
North Section
Carlisle vs. Sunderland
Bolton vs. Sheffield Wednesday
Accrington Stanley vs. West Brom
Newcastle vs. Nottingham Forest
Newport County vs. Leeds (tie to be played at Elland Road at Newport's request)
Stoke vs. Rochdale
Huddersfield vs. Rotherham
Middlesbrough vs. Scunthorpe
Doncaster vs. Hull
Blackburn vs. Burnley
Sheffield United vs. Leicester
Aston Villa vs. Wigan
Barnsley vs. Derby or Grimsby
