The Groupama Stadium hosts the first leg of the knockout-round tie tonight, before the two teams meet at Camp Nou in the return match on March 13.
Tonight will bring just the seventh meeting between these two teams in European competition. Barcelona have won four of the previous six, remaining unbeaten in the process.
The Spanish giants recorded a 6-3 aggregate win when they met in the last-16 stage of the 2008-09 competition, but all four of their other meetings were in the group stages.
Stay tuned for live minute-by-minute coverage of the action below.
LYON: Lopes; Dobois, Denayer, Marcelo, Mendy; Aouar, Ndombele; Terrier, Depay, Traore; Dembele
BARCELONA: Ter Stegen; Semedo, Pique, Lenglet, Alba; Rakitic, Busquets, Roberto; Dembele, L Suarez, Messi