The Spanish giants sealed a fourth La Liga title in the last five seasons with victory over Levante on Saturday, and are now able to turn their full attention to beating last season's Champions League runners-up over two legs.
It promises to be a thrilling contest between two five-time winners of the competition as Barcelona attempt to knock out English opposition for the second round in a row.
Here, Sports Mole rounds up the latest injury and suspension news for Ernesto Valverde's side.
BARCELONA'S INJURY LIST
Type of injury: Knee
Possible return date: Unknown
Rafinha has been sidelined since November after rupturing his cruciate ligament and will not return before the end of the season.
BARCELONA'S SUSPENSION LIST
Barcelona have no players suspended for this match, while all previous yellow cards have been wiped out after the quarter-finals.