Porto's hero of the hour in their Champions League last-16 battle with Juventus, Sergio Oliveira, will play no part in his side's quarter-final first leg with Chelsea on Wednesday evening in Seville.
The 19-goal midfielder bagged a brace to send his side through to the last eight in Turin, but he has now picked up three yellow cards in the tournament and is forced to serve a suspension, so Marko Grujic could partner Mateus Uribe in the middle.
Mehdi Taremi saw red in the second leg of their last-16 tie and will also miss out, so Toni Martinez will hope to partner Moussa Marega after he came off the bench to win the day for Porto deep into added time against Santa Clara last time out.
Otavio is likely to switch over to the left-hand side of Sergio Conceicao's midfield as Jesus Corona comes back in on the right, so Luis Diaz may have to make do with a seat on the bench.
Defensive changes from their narrow win over Santa Clara could see Chancel Mbemba and Zaidu Sanusi return at centre-back and left-back respectively, as Diogo Leite and Nanu are likely to make way.
Malang Sarr is eligible to face his parent club but is unlikely to force his way into the team over 38-year-old Pepe, who was deemed fit enough to start and play the full 90 minutes at the weekend.
Porto possible starting lineup:
Marchesin; Manafa, Pepe, Mbemba, Sanusi; Corona, Grujic, Uribe, Otavio; Marega, Martinez
> Click here to see how Chelsea could line up for this clash
