Alderweireld is the only man who will get the night off as Mourinho confirmed everyone else, including Christian Eriksen, will be involved.
Spurs are hoping to sign Gedson Fernandes on an 18-month loan from Benfica but he would not be eligible to play anyway as players need to have been registered for the original tie.
Assombalonga, who has not played since damaging an ankle against Charlton on December 7, had been expected to join the squad for the trip to the capital but was still struggling in training on Sunday and has been left out of the travelling party.
Keeper Darren Randolph, who underwent a medical at West Ham last week, is fit after a thigh injury but with his proposed transfer yet to be completed, will not figure. Tomas Mejias is set to start once again after playing in the first game.