Both nations are looking to peg back group leaders Scotland, with two wins from the remaining two matches required in order to have any realistic chance of doing so.
Czech Republic lost 1-0 to Germany in an international friendly on Wednesday, with Luca Waldschmidt's goal in the first half proving the difference between the two bordering countries.
Jaroslav Silhavy rested the likes of West Ham United duo Tomas Soucek and Vladimir Coufal, Burnley striker Matej Vydra and Hertha Berlin midfielder Vladimir Darida, with Brescia defender Ales Mateju handed his international debut.
Silhavy's side find themselves four points behind Scotland in the group, but know that finishing ahead of their newly-qualified Euro 2020 group opponents is out of their hands with Steve Clarke's side facing Slovakia and Israel in the coming days.
Indeed, they must beat Israel on Sunday and hope for a favour from their neighbours in order to keep matters alive heading into the final match.
Israel do not have matters in their own hands, either, finding themselves five points behind Scotland, but do have the advantage of facing Scotland in the final match of the group on Wednesday.
Should Willibald Ruttensteiner's side manage to beat Czech Republic, then any slip-up from Scotland in Slovakia would mean that a win for Israel against Clarke's side would guarantee them first place in the group.
That would be sweet revenge indeed for Israel, who were cruelly denied the chance of qualifying for the European Championships for the first time in the nation's history after losing to Scotland on penalties last month.
Instead, World Cup 1970 remains Israel's only ever appearance at a major international tournament. They qualified for that Mexican-based tournament via the Asian confederation, before becoming a permanent member of UEFA in 1994 for safety and political reasons.
Czech Republic Nations League form: WLWL
Czech Republic form (all competitions): WLWWLL
Israel Nations League form: DDLW
Israel form (all competitions): LDDDLW
Bayer Leverkusen striker Patrik Schick was not called up to the squad as he has not played since the last international break due to tearing a muscle.
Ruttensteiner appears to be in a stronger position with no injury isuses, meaning Hoffenheim forward Munas Dabbur and PSV striker Eran Zuhavi should lead the line together.
Zuhavi, who has 24 goals in 57 caps, scored a hat-trick in his nation's 3-2 win at Slovakia in their last match last month.
Czech Republic possible starting lineup:
Vaclik; Coufal, Celustka, Kudela, Novak; Soucek, Kral; Dockal, Darida, Masopust; Vydra
Israel possible starting lineup:
Marciano; Dgani, Tibi, Yeini; Dasa, Natkho, Golasa, Kandil; Solomon, Zahavi, Dabbur
We say: Czech Republic 1-1 Israel
Czech Republic lack cutting edge without star striker Schick, whereas Israel do have two considerable goal threats in the shape of Zahavi and Dabbur.
With both sides needing to win to keep their hopes of finishing above Scotland alive, it could be an entertaining match, so we can see a score draw which will ultimately suit neither nation.