The Reds' 'Invincible' dream was shattered on Saturday as they were well beaten by Watford, ending the joint-longest winning run and second longest unbeaten streak in the history of England's top flight.
While the defeat may not have too much impact on the final Premier League standings, Liverpool must respond quickly if they are to keep their treble hopes alive against Chelsea.
Here, Sports Mole looks at how Jurgen Klopp could line his side up for the contest.
Klopp has used the FA Cup to rotate heavily so far this season, overcoming Everton and Shrewsbury Town with youthful teams while putting his main focus into the Premier League and Champions League.
The strength of Tuesday's opposition may demand a few more senior players starting, although the underwhelming display against Watford could convince Klopp to rest the likes of Mohamed Salah, Roberto Firmino and Sadio Mane.
Youngster Harvey Elliott is away with the Under-19s and so will not continue his run of FA Cup appearances, but Takumi Minamino and Divock Origi, both of whom came off the bench at Vicarage Road, are pushing for starts.
Curtis Jones has scored in both rounds so far and should start again, while fellow youngster Neco Williams starred in the last round against Shrewsbury and could get the nod at right-back if Trent Alexander-Arnold is rested.
Virgil van Dijk may be another to have the night off and, despite Joe Gomez being available after missing the weekend defeat through a minor injury, that could see Joel Matip partner Dejan Lovren at the back.
James Milner is another who is ready to return after being ruled out on Saturday and could provide experience to a youthful side in the middle of the park should Klopp choose to throw him in from the start, but Naby Keita is sidelined again.