The Catalan giants endured a disappointing domestic campaign but remain on course for continental silverware after beating Napoli in the last 16.
In what is a real rarity, though, they will enter a fixture as underdogs against a Bayern side who thrashed Chelsea 7-1 on aggregate in the last round and are unbeaten in 27 matches, winning 26 of those.
Ahead of Friday's much-anticipated clash, Sports Mole rounds up Barca's latest injury and suspension news.
Status: Major doubt
Type of injury: Hamstring
Possible return date: August 14 (vs. Bayern)
Ousmane Dembele has been out for Barca since the end of November with a serious hamstring injury, but he recently returned to training and was a surprise inclusion in their travelling party for this quarter-final.
Type of injury: Knee
Possible return date: Unknown
Samuel Umtiti has not made a first-team outing since the end of June due to knee issues. The centre-back has been given a slight chance of playing again this season, but he is not part of the 26-man squad for the Bayern match.
BARCELONA'S SUSPENSION LIST